Pcr

PCR is a laboratory technique amplifying specific DNA sequences by repeated denaturation and annealing. Target DNA sequences are amplified exponentially in real-time without significant loss of genetic information This method uses primers that bind specifically to the target sequence, facilitating enzymatic synthesis. The polymerase enzyme extends the primer-template complex through nucleotide incorporation and processive replication. PCR products can be used for further analysis, such as sequencing or cloning, to elucidate biological functions